How the Vectaura
Service Works
From your first design to a validated clone.
Build a plasmidCreate an Account and Obtain Assembly Credits
To gain full access to the Vectaura platform, you must first create an account and obtain assembly credits.
Once your account is approved, you will receive an invitation to create a password for the Vectaura tool. Please verify that all account information (such as delivery address) is correct before proceeding.
Design Your Assembly
After access has been granted, you can design and visualize plasmid assemblies in silico using our collection of standardized parts. Selected assemblies can be ordered by spending assembly credits.
Our collection currently contains approximately 1,000 human open reading frames (ORFs) and many additional functional modules. If the cDNA for your protein of interest is not available, it can be added to the collection through DNA synthesis in a compatible donor backbone or by user-provided cloning. For more information, please contact us at [email protected].
As an incentive for community contributions, users who provide a new cDNA module that is made available to all users will receive three complimentary assembly credits.
Assembly Preparation and Shipment
Once an order is placed, the requested assembly reaction will be prepared according to our standard workflow and shipped to the user. Please ensure that your shipment details are correct.
Optionally, local users can collect their assembly reactions at the reception of FSVMII on the 1st floor.
Transformation and Clone Validation
Assemblies are supplied as a 15 µL reaction mixture. Users can transform part of the reaction (typically 5 µL) using their preferred in-house heat-shock protocol or by following our protocol.
After transformation, we recommend plating bacteria on kanamycin-containing agar plates and screening 1–3 colonies by restriction analysis or another suitable validation method. In most cases, analysis of a single colony is sufficient.
Direct inoculation of liquid culture without prior plating is often successful for simple assemblies. Nevertheless, we recommend plating and isolating single colonies to ensure sequence integrity and to obtain high-quality plasmid DNA for downstream applications.
Additional Assembly Reaction
If no correct colonies are obtained from the initial assembly, users may request one additional assembly reaction free of charge, provided that the original protocol was followed and the issue is reported within a reasonable timeframe after receipt.
Provide Feedback
We welcome feedback from all users, whether positive or critical. User experiences and suggestions play an important role in helping us improve both the Vectaura platform and the underlying parts collection.
If you encounter issues, identify missing functionality, or have ideas for new modules or features, please let us know. By sharing your feedback, you help us create a better resource for the community. Note that we will also actively request feedback on parts functionality.
Share your feedback